標(biāo)準(zhǔn)豬血清(無菌過濾、熱滅活)的詳細(xì)資料:
標(biāo)準(zhǔn)豬血清(無菌過濾、熱滅活) 產(chǎn)品介紹:
中文名稱: 標(biāo)準(zhǔn)豬血清(無菌過濾、熱滅活)
規(guī)格: 100ml/500ml/500ml*20
產(chǎn)品編號: YXQ-399
產(chǎn)地:進(jìn)口國產(chǎn)
的產(chǎn)品特點(diǎn):三次無菌過濾,輻射滅菌,批次新,保質(zhì)期久,貼壁效果好,增殖效率高。
遠(yuǎn)慕生物的
血清的保存方法:
(1)長期保存的血清必須儲存于-20℃ - 70℃ 低溫冰箱中.4℃冰箱中保存時間切勿超過1個月.由于血清結(jié)冰時體積會增加約10%,因此,血清在凍入低溫冰箱前,必須預(yù)留一定體積空間,否則易發(fā)生污染或玻璃瓶凍裂.
(2)一般廠商提供的血清為無菌,無需再過濾除菌.如發(fā)現(xiàn)血清有懸浮物,則可將血清加入培養(yǎng)液內(nèi)一起過濾,切勿直接過濾血清.
(3)血清解凍: 瓶裝血清解凍需采用逐步解凍法:-20℃ 至 -70℃ 低溫冰箱中的血清放入4℃冰箱中溶解1天.然后移入室溫,待全部溶解后再分裝.在溶解過程中需不斷輕輕搖晃均勻(小心勿造成氣泡),使溫度與成分均一,減少沉淀的發(fā)生.切勿直接將血清從-20℃進(jìn)入37℃解凍,這樣因溫度改變太大,容易造成蛋白質(zhì)凝集而出現(xiàn)沉淀.
(4)熱滅活是指56℃, 30分鐘加熱已*解凍的血清.加熱過程中須規(guī)則搖晃均勻.此熱處理的目的是使血清中的補(bǔ)體成分(complement)滅活.除非必須,一般不建議作此熱處理,因?yàn)闊崽幚頃斐裳宄恋砦镲@著增多.
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